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rabbit polyclonal antibody against scd1 (Bioss)

Name: rabbit polyclonal antibody against scd1
Brand: Bioss
Rating: 94 (15 reviews)

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Bioss rabbit polyclonal antibody against scd1

<t>SCD1</t> is highly expressed in ovarian cancer tissues compared to normal tissues. ( A, B ) The microarray data for SCD1 mRNA expression in normal ovarian surface epithelial (NOSE) and epithelial ovarian cancer (EOC) tissue samples was obtained from Gene Expression Omnibus (GEO) database, including ( A ) GSE14407 and ( B ) GSE26712. ( C ) Representative IHC images showing SCD1 expression pattern in adjacent normal tissues and ovarian cancer tissues in TMA sections. SCD1 expression was scored as 0, 1+, 2+, or 3 + based on staining intensity. ( D ) Different IHC score of SCD1 protein expression between adjacent normal and ovarian cancer tissues. Values are presented as means ± SEM (** p < 0.01; *** p < 0.001)

Rabbit Polyclonal Antibody Against Scd1, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 15 article reviews

rabbit polyclonal antibody against scd1 - by Bioz Stars, 2026-03

94/100 stars

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1) Product Images from "Stearoyl-CoA desaturase 1 inhibition induces ER stress-mediated apoptosis in ovarian cancer cells"

Article Title: Stearoyl-CoA desaturase 1 inhibition induces ER stress-mediated apoptosis in ovarian cancer cells

Journal: Journal of Ovarian Research

doi: 10.1186/s13048-024-01389-1

SCD1 is highly expressed in ovarian cancer tissues compared to normal tissues. ( A, B ) The microarray data for SCD1 mRNA expression in normal ovarian surface epithelial (NOSE) and epithelial ovarian cancer (EOC) tissue samples was obtained from Gene Expression Omnibus (GEO) database, including ( A ) GSE14407 and ( B ) GSE26712. ( C ) Representative IHC images showing SCD1 expression pattern in adjacent normal tissues and ovarian cancer tissues in TMA sections. SCD1 expression was scored as 0, 1+, 2+, or 3 + based on staining intensity. ( D ) Different IHC score of SCD1 protein expression between adjacent normal and ovarian cancer tissues. Values are presented as means ± SEM (** p < 0.01; *** p < 0.001)

Figure Legend Snippet: SCD1 is highly expressed in ovarian cancer tissues compared to normal tissues. ( A, B ) The microarray data for SCD1 mRNA expression in normal ovarian surface epithelial (NOSE) and epithelial ovarian cancer (EOC) tissue samples was obtained from Gene Expression Omnibus (GEO) database, including ( A ) GSE14407 and ( B ) GSE26712. ( C ) Representative IHC images showing SCD1 expression pattern in adjacent normal tissues and ovarian cancer tissues in TMA sections. SCD1 expression was scored as 0, 1+, 2+, or 3 + based on staining intensity. ( D ) Different IHC score of SCD1 protein expression between adjacent normal and ovarian cancer tissues. Values are presented as means ± SEM (** p < 0.01; *** p < 0.001)

Techniques Used: Microarray, Expressing, Staining

SCD1 expression is significantly elevated in EOC cell lines compared to NOSE cell lines. ( A ) The mRNA expression levels of SCD1 in EOC cell lines and NOSE cell lines. SCD1 mRNA levels were analyzed by qRT-PCR analysis and normalized to GAPDH mRNA levels. ( B ) The protein expression levels of SCD1 in EOC cell lines and NOSE cell lines were detected by western blot analysis. ( C ) SCD1 protein levels were quantified by densitometry using ImageJ software followed by normalization to GAPDH protein levels. Data are presented as the mean ± SEM of three independent experiments

Figure Legend Snippet: SCD1 expression is significantly elevated in EOC cell lines compared to NOSE cell lines. ( A ) The mRNA expression levels of SCD1 in EOC cell lines and NOSE cell lines. SCD1 mRNA levels were analyzed by qRT-PCR analysis and normalized to GAPDH mRNA levels. ( B ) The protein expression levels of SCD1 in EOC cell lines and NOSE cell lines were detected by western blot analysis. ( C ) SCD1 protein levels were quantified by densitometry using ImageJ software followed by normalization to GAPDH protein levels. Data are presented as the mean ± SEM of three independent experiments

Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Software

SCD1 inhibition reduces cancer cell proliferation without cytotoxic effect on normal cells. ( A ) PA-1 and SKOV-3 cells were transfected with SCD1 siRNA (100 nM) or scrambled siRNA (100 nM) as a negative control. After 72 h, the ablation of SCD1 protein was determined by western blot analysis. GAPDH was used as a loading control. ( B ) PA-1 and SKOV-3 cells were transfected with SCD1 siRNA (100 nM). At 72 h post-transfection, cell viability was analyzed using MTT assay. ( C ) PA-1 and SKOV-3 cells were treated with various concentrations (0, 5, 10, 20, 50, and 100 nM) of CAY10566 or DMSO (solvent control) for 24–48 h. Cell viability was measured by MTT assay. ( D ) Bright field microscopy images of patient-derived ovarian cancer organoids A209 (top) and A220 (bottom) after 28 days of culture. Scale bar = 500 μm. ( E ) Ovarian cancer organoids A209 and A220 were treated with varying concentrations (0, 10, 20, 50, and 100 nM) of CAY10566 or DMSO (solvent control) for 24–48 h. Cell viability was assessed using the organoid MTT assay. ( F ) CAY10566 were treated in NOSE cell lines (IOSE385 and SNU3236) and peripheral blood mononuclear cells (PBMCs) with different concentrations of CAY10566. After 24–48 h, cell viability was examined by MTT assay. All data were described as mean ± SEM of three independent experiments (** p < 0.01; *** p < 0.001; **** p < 0.0001)

Figure Legend Snippet: SCD1 inhibition reduces cancer cell proliferation without cytotoxic effect on normal cells. ( A ) PA-1 and SKOV-3 cells were transfected with SCD1 siRNA (100 nM) or scrambled siRNA (100 nM) as a negative control. After 72 h, the ablation of SCD1 protein was determined by western blot analysis. GAPDH was used as a loading control. ( B ) PA-1 and SKOV-3 cells were transfected with SCD1 siRNA (100 nM). At 72 h post-transfection, cell viability was analyzed using MTT assay. ( C ) PA-1 and SKOV-3 cells were treated with various concentrations (0, 5, 10, 20, 50, and 100 nM) of CAY10566 or DMSO (solvent control) for 24–48 h. Cell viability was measured by MTT assay. ( D ) Bright field microscopy images of patient-derived ovarian cancer organoids A209 (top) and A220 (bottom) after 28 days of culture. Scale bar = 500 μm. ( E ) Ovarian cancer organoids A209 and A220 were treated with varying concentrations (0, 10, 20, 50, and 100 nM) of CAY10566 or DMSO (solvent control) for 24–48 h. Cell viability was assessed using the organoid MTT assay. ( F ) CAY10566 were treated in NOSE cell lines (IOSE385 and SNU3236) and peripheral blood mononuclear cells (PBMCs) with different concentrations of CAY10566. After 24–48 h, cell viability was examined by MTT assay. All data were described as mean ± SEM of three independent experiments (** p < 0.01; *** p < 0.001; **** p < 0.0001)

Techniques Used: Inhibition, Transfection, Negative Control, Western Blot, Control, MTT Assay, Solvent, Microscopy, Derivative Assay

MUFA/SFA ratio alteration induced by SCD1 inhibition leads to ER stress-mediated apoptosis. ( A ) PA-1 and SKOV-3 cells were treated with the IC50 value of CAY10566 for PA-1 cells (20 nM) for 48 h. Desaturase activity of SCD1 was estimated as the ratios of palmitoleic acid (C16:1n7) to palmitic acid (C16:0) and oleic acid (C18:1n9c) to stearic acid (C18:0) using gas chromatography. The statistical analysis was conducted using a two-way ANOVA followed by Šídák’s multiple comparisons test. ( B ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The percentage of apoptotic cells was determined by flow cytometry analysis using Annexin V-FITC and PI staining. ( C ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The expression levels of apoptosis marker proteins, PARP and cleaved caspase-3, were detected by western blot analysis. GAPDH was used as a loading control. ( D ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The expression levels of ER stress marker proteins, p-PERK, IRE1α, ATF4, and CHOP, were examined by western blot analysis. GAPDH was used as a loading control. All data were described as mean ± SEM of three independent experiments (** p < 0.01; *** p < 0.001; **** p < 0.0001)

Figure Legend Snippet: MUFA/SFA ratio alteration induced by SCD1 inhibition leads to ER stress-mediated apoptosis. ( A ) PA-1 and SKOV-3 cells were treated with the IC50 value of CAY10566 for PA-1 cells (20 nM) for 48 h. Desaturase activity of SCD1 was estimated as the ratios of palmitoleic acid (C16:1n7) to palmitic acid (C16:0) and oleic acid (C18:1n9c) to stearic acid (C18:0) using gas chromatography. The statistical analysis was conducted using a two-way ANOVA followed by Šídák’s multiple comparisons test. ( B ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The percentage of apoptotic cells was determined by flow cytometry analysis using Annexin V-FITC and PI staining. ( C ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The expression levels of apoptosis marker proteins, PARP and cleaved caspase-3, were detected by western blot analysis. GAPDH was used as a loading control. ( D ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The expression levels of ER stress marker proteins, p-PERK, IRE1α, ATF4, and CHOP, were examined by western blot analysis. GAPDH was used as a loading control. All data were described as mean ± SEM of three independent experiments (** p < 0.01; *** p < 0.001; **** p < 0.0001)

Techniques Used: Inhibition, Activity Assay, Gas Chromatography, Transfection, Flow Cytometry, Staining, Expressing, Marker, Western Blot, Control

Addition of exogenous oleic acid rescued ER stress-mediated apoptosis triggered by SCD1 inhibition. ( A ) PA-1 cells were treated with CAY10566 (20 nM) with oleic acid-BSA (10 mM) or fatty acid-free BSA (10 µM) as vehicle control for 48 h. Cell viability was analyzed by MTT assay. Results were presented as the percentage of total cell number compared to DMSO-treated control. ( B ) PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 µM) for 48 h. The percentage of apoptotic cells was calculated by flow cytometry analysis using Annexin V-FITC and PI staining. ( C ) Inhibition of CAY10566-mediated PARP and caspase-3 cleavage by supplementation with oleic acid. PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 mM) for 48 h. The expression levels of cleaved PARP and caspase-3 were assessed by western blot analysis. GAPDH was used as a loading control. ( D ) PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 µM) for 48 h. The expression levels of P-PERK, IRE1α, ATF4, and CHOP were determined by western blot analysis. GAPDH was used as a loading control. All data were described as mean ± SEM of three independent experiments (* p < 0.05; ** p < 0.01; *** p < 0.001)

Figure Legend Snippet: Addition of exogenous oleic acid rescued ER stress-mediated apoptosis triggered by SCD1 inhibition. ( A ) PA-1 cells were treated with CAY10566 (20 nM) with oleic acid-BSA (10 mM) or fatty acid-free BSA (10 µM) as vehicle control for 48 h. Cell viability was analyzed by MTT assay. Results were presented as the percentage of total cell number compared to DMSO-treated control. ( B ) PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 µM) for 48 h. The percentage of apoptotic cells was calculated by flow cytometry analysis using Annexin V-FITC and PI staining. ( C ) Inhibition of CAY10566-mediated PARP and caspase-3 cleavage by supplementation with oleic acid. PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 mM) for 48 h. The expression levels of cleaved PARP and caspase-3 were assessed by western blot analysis. GAPDH was used as a loading control. ( D ) PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 µM) for 48 h. The expression levels of P-PERK, IRE1α, ATF4, and CHOP were determined by western blot analysis. GAPDH was used as a loading control. All data were described as mean ± SEM of three independent experiments (* p < 0.05; ** p < 0.01; *** p < 0.001)

Techniques Used: Inhibition, Control, MTT Assay, Flow Cytometry, Staining, Expressing, Western Blot

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Journal: Journal of Ovarian Research

Article Title: Stearoyl-CoA desaturase 1 inhibition induces ER stress-mediated apoptosis in ovarian cancer cells

doi: 10.1186/s13048-024-01389-1

Figure Lengend Snippet: SCD1 is highly expressed in ovarian cancer tissues compared to normal tissues. ( A, B ) The microarray data for SCD1 mRNA expression in normal ovarian surface epithelial (NOSE) and epithelial ovarian cancer (EOC) tissue samples was obtained from Gene Expression Omnibus (GEO) database, including ( A ) GSE14407 and ( B ) GSE26712. ( C ) Representative IHC images showing SCD1 expression pattern in adjacent normal tissues and ovarian cancer tissues in TMA sections. SCD1 expression was scored as 0, 1+, 2+, or 3 + based on staining intensity. ( D ) Different IHC score of SCD1 protein expression between adjacent normal and ovarian cancer tissues. Values are presented as means ± SEM (** p < 0.01; *** p < 0.001)

Article Snippet: IHC staining was conducted using a rabbit polyclonal antibody against SCD1 (1:200, bs-3787R, Bioss, Woburn, MA).

Techniques: Microarray, Expressing, Staining

Journal: Journal of Ovarian Research

Article Title: Stearoyl-CoA desaturase 1 inhibition induces ER stress-mediated apoptosis in ovarian cancer cells

doi: 10.1186/s13048-024-01389-1

Figure Lengend Snippet: SCD1 expression is significantly elevated in EOC cell lines compared to NOSE cell lines. ( A ) The mRNA expression levels of SCD1 in EOC cell lines and NOSE cell lines. SCD1 mRNA levels were analyzed by qRT-PCR analysis and normalized to GAPDH mRNA levels. ( B ) The protein expression levels of SCD1 in EOC cell lines and NOSE cell lines were detected by western blot analysis. ( C ) SCD1 protein levels were quantified by densitometry using ImageJ software followed by normalization to GAPDH protein levels. Data are presented as the mean ± SEM of three independent experiments

Article Snippet: IHC staining was conducted using a rabbit polyclonal antibody against SCD1 (1:200, bs-3787R, Bioss, Woburn, MA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Software

Journal: Journal of Ovarian Research

Article Title: Stearoyl-CoA desaturase 1 inhibition induces ER stress-mediated apoptosis in ovarian cancer cells

doi: 10.1186/s13048-024-01389-1

Figure Lengend Snippet: SCD1 inhibition reduces cancer cell proliferation without cytotoxic effect on normal cells. ( A ) PA-1 and SKOV-3 cells were transfected with SCD1 siRNA (100 nM) or scrambled siRNA (100 nM) as a negative control. After 72 h, the ablation of SCD1 protein was determined by western blot analysis. GAPDH was used as a loading control. ( B ) PA-1 and SKOV-3 cells were transfected with SCD1 siRNA (100 nM). At 72 h post-transfection, cell viability was analyzed using MTT assay. ( C ) PA-1 and SKOV-3 cells were treated with various concentrations (0, 5, 10, 20, 50, and 100 nM) of CAY10566 or DMSO (solvent control) for 24–48 h. Cell viability was measured by MTT assay. ( D ) Bright field microscopy images of patient-derived ovarian cancer organoids A209 (top) and A220 (bottom) after 28 days of culture. Scale bar = 500 μm. ( E ) Ovarian cancer organoids A209 and A220 were treated with varying concentrations (0, 10, 20, 50, and 100 nM) of CAY10566 or DMSO (solvent control) for 24–48 h. Cell viability was assessed using the organoid MTT assay. ( F ) CAY10566 were treated in NOSE cell lines (IOSE385 and SNU3236) and peripheral blood mononuclear cells (PBMCs) with different concentrations of CAY10566. After 24–48 h, cell viability was examined by MTT assay. All data were described as mean ± SEM of three independent experiments (** p < 0.01; *** p < 0.001; **** p < 0.0001)

Article Snippet: IHC staining was conducted using a rabbit polyclonal antibody against SCD1 (1:200, bs-3787R, Bioss, Woburn, MA).

Techniques: Inhibition, Transfection, Negative Control, Western Blot, Control, MTT Assay, Solvent, Microscopy, Derivative Assay

Journal: Journal of Ovarian Research

Article Title: Stearoyl-CoA desaturase 1 inhibition induces ER stress-mediated apoptosis in ovarian cancer cells

doi: 10.1186/s13048-024-01389-1

Figure Lengend Snippet: MUFA/SFA ratio alteration induced by SCD1 inhibition leads to ER stress-mediated apoptosis. ( A ) PA-1 and SKOV-3 cells were treated with the IC50 value of CAY10566 for PA-1 cells (20 nM) for 48 h. Desaturase activity of SCD1 was estimated as the ratios of palmitoleic acid (C16:1n7) to palmitic acid (C16:0) and oleic acid (C18:1n9c) to stearic acid (C18:0) using gas chromatography. The statistical analysis was conducted using a two-way ANOVA followed by Šídák’s multiple comparisons test. ( B ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The percentage of apoptotic cells was determined by flow cytometry analysis using Annexin V-FITC and PI staining. ( C ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The expression levels of apoptosis marker proteins, PARP and cleaved caspase-3, were detected by western blot analysis. GAPDH was used as a loading control. ( D ) PA-1 cells were transfected with SCD1 siRNA (100 nM) or treated with CAY10566 (20 nM) for 48 h. The expression levels of ER stress marker proteins, p-PERK, IRE1α, ATF4, and CHOP, were examined by western blot analysis. GAPDH was used as a loading control. All data were described as mean ± SEM of three independent experiments (** p < 0.01; *** p < 0.001; **** p < 0.0001)

Article Snippet: IHC staining was conducted using a rabbit polyclonal antibody against SCD1 (1:200, bs-3787R, Bioss, Woburn, MA).

Techniques: Inhibition, Activity Assay, Gas Chromatography, Transfection, Flow Cytometry, Staining, Expressing, Marker, Western Blot, Control

Journal: Journal of Ovarian Research

Article Title: Stearoyl-CoA desaturase 1 inhibition induces ER stress-mediated apoptosis in ovarian cancer cells

doi: 10.1186/s13048-024-01389-1

Figure Lengend Snippet: Addition of exogenous oleic acid rescued ER stress-mediated apoptosis triggered by SCD1 inhibition. ( A ) PA-1 cells were treated with CAY10566 (20 nM) with oleic acid-BSA (10 mM) or fatty acid-free BSA (10 µM) as vehicle control for 48 h. Cell viability was analyzed by MTT assay. Results were presented as the percentage of total cell number compared to DMSO-treated control. ( B ) PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 µM) for 48 h. The percentage of apoptotic cells was calculated by flow cytometry analysis using Annexin V-FITC and PI staining. ( C ) Inhibition of CAY10566-mediated PARP and caspase-3 cleavage by supplementation with oleic acid. PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 mM) for 48 h. The expression levels of cleaved PARP and caspase-3 were assessed by western blot analysis. GAPDH was used as a loading control. ( D ) PA-1 cells were treated with CAY10566 (20 nM) with or without oleic acid-BSA (10 µM) for 48 h. The expression levels of P-PERK, IRE1α, ATF4, and CHOP were determined by western blot analysis. GAPDH was used as a loading control. All data were described as mean ± SEM of three independent experiments (* p < 0.05; ** p < 0.01; *** p < 0.001)

Article Snippet: IHC staining was conducted using a rabbit polyclonal antibody against SCD1 (1:200, bs-3787R, Bioss, Woburn, MA).

Techniques: Inhibition, Control, MTT Assay, Flow Cytometry, Staining, Expressing, Western Blot

List of primer sequences used for RT-qPCR.

Journal: PLoS ONE

Article Title: Uromodulin Retention in Thick Ascending Limb of Henle's Loop Affects SCD1 in Neighboring Proximal Tubule: Renal Transcriptome Studies in Mouse Models of Uromodulin-Associated Kidney Disease

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: List of primer sequences used for RT-qPCR.

Article Snippet: The following primary antibodies were used: rat monoclonal antibody against mouse ANGPTL7 (clone 538401; R&D Systems), rabbit monoclonal antibody against GAPDH (#2118, Cell Signaling), rabbit polyclonal antibody against mouse SCD1 (#2438, Cell Signaling).

Techniques:

$( A ) SCD1 was detected in the cytoplasmic compartment selectively of proximal tubular cells (in the straight S3 segment). In the kidney of the homozygous Umod C93F mutant mouse, SCD1 appeared to be abundant in a larger fraction of proximal tubular cells compared to the kidney of the wild-type mouse, and the average staining intensity of SCD1 positive cells appeared to be more prominent. Age of mice analyzed: four months. Umod wt : wild-type mouse; Umod C93F : homozygous Umod C93F mutant mouse. Uromodulin immunohistochemistry enabled identification of TALH segments. Proximal tubule segment are morphologically characterized by luminal microvilli. Chromogen: DAB for SCD1, Vector RED for UMOD; nuclear staining: hemalum. ( B ) Protein abundance of SCD1 in whole kidney lysate of homozygous Umod mutant mice of both lines was increased compared to wild-type mice. Signal intensities of SCD1 were corrected for GAPDH signal intensities of the same PVDF-membrane. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. One-way-ANOVA with Tukey's Multiple Comparison Post hoc Test: p vs. wild-type, **, p <0.01; ***, p<0.001. Age of mice analyzed: four months.$

Journal: PLoS ONE

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: ( A ) SCD1 was detected in the cytoplasmic compartment selectively of proximal tubular cells (in the straight S3 segment). In the kidney of the homozygous Umod C93F mutant mouse, SCD1 appeared to be abundant in a larger fraction of proximal tubular cells compared to the kidney of the wild-type mouse, and the average staining intensity of SCD1 positive cells appeared to be more prominent. Age of mice analyzed: four months. Umod wt : wild-type mouse; Umod C93F : homozygous Umod C93F mutant mouse. Uromodulin immunohistochemistry enabled identification of TALH segments. Proximal tubule segment are morphologically characterized by luminal microvilli. Chromogen: DAB for SCD1, Vector RED for UMOD; nuclear staining: hemalum. ( B ) Protein abundance of SCD1 in whole kidney lysate of homozygous Umod mutant mice of both lines was increased compared to wild-type mice. Signal intensities of SCD1 were corrected for GAPDH signal intensities of the same PVDF-membrane. Mean of protein abundance of wild-type mice was set on a value of 1 [mean (wild-type) = 1]. One-way-ANOVA with Tukey's Multiple Comparison Post hoc Test: p vs. wild-type, **, p <0.01; ***, p<0.001. Age of mice analyzed: four months.

Techniques: Mutagenesis, Staining, Immunohistochemistry, Plasmid Preparation, Quantitative Proteomics, Membrane, Comparison

Increased Scd1 transcript abundance in kidneys of homozygous Slc12a1 I299T mutant mice compared to the kidneys of littermate controls were detected by RT-qPCR analysis. Data are shown as scatter dot plot with mean (n = 5 per group). Age of mice analyzed: three months. Student's t test: p vs. wild-type, *, p <0.05.

Journal: PLoS ONE

doi: 10.1371/journal.pone.0113125

Figure Lengend Snippet: Increased Scd1 transcript abundance in kidneys of homozygous Slc12a1 I299T mutant mice compared to the kidneys of littermate controls were detected by RT-qPCR analysis. Data are shown as scatter dot plot with mean (n = 5 per group). Age of mice analyzed: three months. Student's t test: p vs. wild-type, *, p <0.05.

Techniques: Mutagenesis, Quantitative RT-PCR

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